Initial Rate Studies of Adenylosuccinate Synthetase with Product and Competitive Inhibitors
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چکیده
منابع مشابه
Variations in the response of mouse isozymes of adenylosuccinate synthetase to inhibitors of physiological relevance.
Vertebrates have acidic and basic isozymes of adenylosuccinate synthetase, which participate in the first committed step of de novo AMP biosynthesis and/or the purine nucleotide cycle. These isozymes differ in their kinetic properties and N-leader sequences, and their regulation may vary with tissue type. Recombinant acidic and basic synthetases from mouse, in the presence of active site ligand...
متن کاملpostnatal studies of bats (pipistrellus kuhlii and miniopterus schreibersii) & histomorphology and histochemistry studies of organs and diseases of (neurergus microspilotus and n. kaiseri)
1. to determine whether difference in birth body mass influenced growth performance in pipistrellus kuhlii we studied a total of 12 captive-born neonates. bats were assigned to two body mass groups: light birth body mass (lbw: 0.89 ± 0.05, n=8) and heavy birth body mass (hbw: 1.35 ± 0.08, n=4). heavier body mass at birth was associated with rapid postnatal growth (body mass and forearm length) ...
Cloning and characterization of the cDNA encoding human adenylosuccinate synthetase.
Adenylosuccinate synthetase (AS) catalyzes the first committed step in the conversion of IMP to AMP. A cDNA was isolated from a human liver library which encodes a protein of 455 amino acids (M(r) of 49,925). Alignments of human, mouse, Dictyostelium discoideum and E. coli AS sequences identify a number of invariant residues which are likely to be important for structure and/or catalysis. The h...
متن کاملReactions of phosphorothioate compounds catalyzed by adenylosuccinate synthetase. Steady state and pre-steady state kinetic studies.
Kinetic studies of the reactions catalyzed by adenylosuccinate synthetase with phosphorothioate derivatives of substrates for the forward and reverse reactions provide evidence for an intermediate on the reaction pathway. Guanosine-5’-0-(3-thiotriphosphate) (GTPyS) replaces GTP with a 12.5-fold reduction in the maximal velocity (at 22”C), although the K, for GTPyS is 3.5-fold lower than the K,,...
متن کاملSynergistic inhibition of adenylosuccinate synthetase by nitrate and GDP.
Adenylosuccinate synthetase (IMP: L-aspartate ligase (GDP), EC 6.3.4.4) occupies a central position in the biosynthesis of AMP and in purine nucleotide cycling [ 11. Because of the enzyme’s unique function in adenine nucleotide synthesis, regulation and inhibition of this enzyme are of potential significance in control of cellular proliferation. Recent studies have shown that the planar nitrate...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1969
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)36425-6